Biomolecular Synthesis - Scientific Background

We synthesize peptides using solid phase peptide synthesis (SPPS) with Fmoc chemistry. The peptides are synthesized from the C-terminus by stepwise addition of amino acids. Initially, the first Fmoc-amino acid is attached to a support resin via an acid labile linker. After deprotection of Fmoc by treatment with piperidine, the second Fmoc-amino acid is coupled via in situ activation.

After the desired peptide is synthesized, the resin bound peptide is deprotected and cleaved from the resin by TFA treatment. Completed peptides are purified with preparative reverse phase HPLC.  All peptides are provided with mass spectrometric and UPLC analysis, to confirm the molecule mass and purity.